International Journal of Research in Pharmacy and Allied Science

ISSN: 2583-6544   |  Issues per year: Monthly  |   Indexed In: Google Scholar, CORE, ScienceOpen, Researchgate

ISSN:2583-6544

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RP-HPLC Method Development and Validation on Nortriptyline Hydrochloride Tablets

Author(s): Dipak Narsing Shinde1, Dr.Balaji M. Shetkar2, Dr. Kranti L Satpute3

Email(s): 1dipakshinde7465@gmail.com

Address:

    Dayanand College of Pharmacy, Latur, Maharashtra, India..

Published In:   Volume - 2,      Issue - 4,     Year - 2023

DOI: Not Available

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ABSTRACT:
RP-HPLC method was developed for Nortriptyline Hydrochloride in the tablet dosage form. The chromatographic separation was performed on InfinityLab Poroshell 120 EC-C18 (150 mm×4.6 mm, 4 µm) column, with a mobile phase, Acetonitrile with 0.1% OPA [pH 5.0] in the ratio of 50:50 v/v at the flow rate of 1.0 ml/min. The detection was carried out at 250 nm using PDA detector. The retention time of NH was found to be 2.24 min with a run time of 10 min. As per ICH Q2R1 guideline, the method was validated for linearity, accuracy, precision and robustness. The linearity of NH was found in the range of 10-50 µg/ml. The correlation coefficient for NH was found 0.99. This demonstrates that the developed method is economical, simple, precise, rapid, selective and accurate for the estimation of NH in tablet dosage form and it can be successfully utilized for the quantitative analysis of pharmaceutical tablet dosage formulations containing Nortriptyline hydrochloride.

Keywords:

Cite this article:
Dipak Narsing Shinde, Dr.Balaji M. Shetkar, Dr. Kranti L Satpute. RP-HPLC Method Development and Validation on Nortriptyline Hydrochloride Tablets. IJRPAS, May-June 2023; 2(3): 1-9.


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