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Author(s): Gautam P. Vadnere*1, Md. Rageeb Md. Usman2, Shreya C. Jain3, Swati Turkhade4, Anuja Patil5, Karuna Patil6

Email(s): 1rageebshaikh@gmail.com

Address:

    Smt. Sharadchandrika Suresh Patil College of Pharmacy, Chopda, Maharashtra, India

Published In:   Volume - 4,      Issue - 5,     Year - 2025

DOI: https://doi.org/10.71431/IJRPAS.2025.4501  

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ABSTRACT:
Ocimum tenuiflorum (Holy Basil) is an important medicinal herb extensively employed across traditional healing systems like Ayurveda, Siddha, and Unani due to its wide range of therapeutic benefits. In the present research, essential oils were obtained from the entire plant including leaves, stems, flowers, roots, and seeds through steam distillation. The extracted oil underwent detailed evaluation using qualitative and quantitative phytochemical screening, physicochemical tests, and gas chromatography with flame ionization detection (GC-FID). Preliminary phytochemical tests confirmed the presence of key constituents such as alkaloids, flavonoids, phenolics, terpenoids, and glycosides. Quantitative analysis revealed total phenolic content of 1.61%, flavonoids at 1.56%, and alkaloids at 0.91%. GC-FID analysis identified 24 chemical constituents in the essential oil, with eugenol being predominant (83.08%), followed by citronellol, sabinene, and carvacrol. These compounds are known to exhibit significant antimicrobial, antioxidant, and anti-inflammatory properties. This study supports the pharmacological relevance of O. tenuiflorum and offers a scientific basis for its traditional applications and further exploration in pharmaceutical industries.

Cite this article:
Gautam P. Vadnere*, Md. Rageeb Md. Usman, Shreya C. Jain, Swati Turkhade, Anuja Patil, Karuna Patil. Gas Chromatographic Profiling and Characterization of Essential Oils from Ocimum tenuiflorum. IJRPAS, May 2025; 4 (5): 1-12DOI: https://doi.org/https://doi.org/10.71431/IJRPAS.2025.4501


1.                  Farooqi AA, Sreeramu BS. Cultivation of Medicinal and Aromatic Crops. 2nd ed. Hyderabad: Universities Press (India) Pvt. Ltd; 2004. p. 529–34.

2.                  Sirkar NN. Pharmacological basis of Ayurvedic therapeutics. In: Atal CK, Kapoor BM, editors. Cultivation and utilization of medicinal plants. New Delhi: PID CSIR; 1982. p. 515–7.

3.                  Prakash P, Gupta N. Therapeutic use of Ocimum sanctum Linn (Tulsi) with note on eugenol and its pharmacological action: A short review. Indian J Physiol Pharmacol. 2005;49(2):125–31.

4.                  Singh S, Taneja M, Majumdar DK. Biological activities of Ocimum sanctum L. fixed oil – An overview. Indian J Exp Biol. 2007;45(5):403–12.

5.                  Singh S, Majumdar DK. Evaluation of the gastric antiulcer activity of fixed oil of Ocimum sanctum (Holy Basil). J Ethnopharmacol. 1999;65(1):13–9.

6.                  Pingale SS, Firke NP, Markandeya AG. Therapeutic activities of Ocimum tenuiflorum accounted in last decade: A review. J Pharm Res. 2012;5(4):2215–20.

7.                  Bhattarai K, Bhattarai R, Pandey RD, Paudel B, Bhattarai HD. Comprehensive review of the phytochemical constituents and bioactivities of Ocimum tenuiflorum. Sci World J. 2024;2024:8895039.

8.                  Joshi RK, Hoti SL. Chemical composition of the essential oil of Ocimum tenuiflorum L. (Krishna Tulsi) from North West Karnataka, India. Plant Sci Today. 2014;1(3):99–102.

9.                  Nahak G, Mishra RC, Sahu RK. Taxonomic distribution, medicinal properties and drug development potentiality of Ocimum (Tulsi). Drug Invent Today. 2011;3(6):95–113.

10.              Borah R, Biswas SP. Tulsi (Ocimum sanctum), excellent source of phytochemicals. Int J Environ Agric Biotechnol. 2018;3(5):2893–7. doi:10.22161/ijeab/3.5.21

11.              Masango P. Cleaner production of essential oils by steam distillation. J Clean Prod. 2005;13(8):833–9.

12.              Cassel E, Vargas RMF, Martinez N, Lorenzo D, Dellacassa E. Steam distillation modeling for essential oil extraction process. Ind Crops Prod. 2009;29(1):171–6.

13.              Machado CA, Oliveira FO, de Andrade MA, Hodel KVS, Lepikson H, Machado BAS. Steam distillation for essential oil extraction: An evaluation of technological advances based on an analysis of patent documents. Sustainability. 2022;14(12):7119.

14.              Sahraoui N, Vian MA, Bornard I, Boutekedjiret C, Chemat F. Improved microwave steam distillation apparatus for isolation of essential oils: Comparison with conventional steam distillation. J Chromatogr A. 2008;1210(2):229–33.

15.              Mindaryani A, Rahayu SS. Essential oil from extraction and steam distillation of Ocimum basilicum. In: WCECS 2007; 2007 Oct 24–26; San Francisco, USA. Hong Kong: IAENG; 2007. ISBN: 978-988-98671-6-4.

16.              Indian Pharmacopoeia. Vol. 3. Ghaziabad: Indian Pharmacopoeia Commission; 2018.

17.              Kokate CK, Purohit AP, Gokhale SB. Pharmacognosy. 47th ed. Pune: Nirali Publications; 2015.

18.              Ayurvedic Pharmacopoeia of India. Vol. 1. 1st ed. Ghaziabad: Ministry of AYUSH, Government of India; 2022.

19.              George D. Characterization and evaluation of Tulsi (Ocimum tenuiflorum L.) [MSc thesis]. Kerala, India: College of Agriculture, Vellanikkara; 2021.

20.              Bano N, Ahmed A, Tanveer M, Khan GM, Ansari MT. Pharmacological evaluation of Ocimum sanctum. J Bioequiv Availab. 2017;9(3):387–92.

21.              Boham BA, Kocipai AR. Flavonoids and condensed tannins from leaves of Hawaiian Vaccinium vaticulatum and V. calycinum. Pac Sci. 1994;48:458–63.

22.              Cohen MM. Tulsi – Ocimum sanctum: A herb for all reasons. J Ayurveda Integr Med. 2014;5(4):251–9.

23.              Sharma P, Upadhyaya K. Characteristic features and comparative analysis of essential oil composition of selected genus of Ocimum sanctum L. through GC-MS. J Vector Borne Dis. 2023;60(1):94–100.

24.              Mukherjee S, Chandrakar M, Gupta P, Khan A, Pal R, Dwivedi A, et al. A two-stage extraction model for simultaneous extraction of essential oil and phenolics from tulsi leaves: Implementing a blended mode microwave hydrodiffusion and gravity (MHG) model. Sustain Food Technol. 2024;2:1686–96.

25.              Gnanamurthy P, Narasimhan MK, Sabarathinam S. GC-MS analysis of an ethanolic extract of Ocimum species: A network pharmacology analysis insight towards obesity. Future Sci OA. 2024;10(1):FSO940.

26.              Chowdhury T, Mandal A, Roy SC, De Sarker D. Diversity of the genus Ocimum (Lamiaceae) through morpho-molecular (RAPD) and chemical (GC–MS) analysis. J Genet Eng Biotechnol. 2017;15(1):275–86.

27.              Sharma P. Characteristic features and comparative analysis of essential oil composition of selected species of Ocimum sanctum L. through GCMS [Preprint]. 2021 Jan 16. doi:10.21203/rs.3.rs-139700/v1.

28.              Marriott PJ, Shellie R, Cornwell C. Gas chromatographic technologies for the analysis of essential oils. J Chromatogr A. 2001;936(1–2):1–22.

29.              Cagliero C, Bicchi C, Marengo A. Gas chromatography of essential oil: State-of-the-art, recent advances, and perspectives. J Sep Sci. 2022;45(1):94–112.

30.              Adams RP. Identification of Essential Oil Components by Gas Chromatography/Mass Spectrometry. 4th ed. Carol Stream, IL: Allured Publishing; 2007.

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