ABSTRACT:
UV-Visible spectrophotometry is a widely employed analytical technique for the quantitative determination of various chemical substances in pharmaceutical, biological, and environmental samples. The method development process involves optimizing parameters such as wavelength selection, solvent compatibility, and calibration curve establishment to ensure accuracy, precision, and reproducibility. This study focuses on the development and validation of a UV-Visible spectrophotometric method for [specific analyte] in [specific matrix, e.g., pharmaceutical formulations]. The analytical method was developed by identifying the maximum absorption wavelength (λmax) of the analyte and optimizing the sample preparation procedure. A linear relationship was observed between absorbance and concentration within the range of [specific concentration range], with a correlation coefficient (R²) of [specific value]. Method validation was performed following ICH guidelines, assessing parameters such as linearity, accuracy, precision, limit of detection (LOD), and limit of quantification (LOQ). The results demonstrated the method's robustness and suitability for routine quality control analysis. This study highlights the effectiveness of UV-Visible spectrophotometry in achieving reliable and efficient analytical solutions for various applications.
Cite this article:
Mr. Shrinivas Kapale, Mr. Gopal Lohiya, Dr.Kranti Satpute. Review on Analytical Method Development & Validation by Using UV-Visible Spectroscopy. IJRPAS, 2024; 3(6): 147-159.
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