Author(s):
Vaibhav Jain1, Rajkumar Chauhan2, B. P. Nagori3, Sabir Anis4, Ranjan Kumar Singh5
Email(s):
1vaibhavs.jain96@gmail.com
Address:
1Lachoo Memorial College of Science & Technology (Autonomous) Pharmacy Wing, Jodhpur - 342001, Rajasthan, India
2Lachoo Memorial College of Science & Technology (Autonomous) Pharmacy Wing, Jodhpur - 342001, Rajasthan, India
3Lachoo Memorial College of Science & Technology (Autonomous) Pharmacy Wing, Jodhpur - 342001, Rajasthan, India
4Mualana Azad University, Jodhpur, Rajasthan, India
5School of pharmacy, Dept. of Pharmacology, Neemrana, Alwar, Rajasthan-341705
Published In:
Volume - 3,
Issue - 3,
Year - 2024
DOI:
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ABSTRACT:
A simple, novel, sensitive, and precise validated spectrophotometric method was developed for the simultaneous determination of Mebendazole (MBZ) and Levamisole Hydrochloride (LVM) in its tablet formulation. 1% H2SO4 in methanol was selected as a common solvent for the estimation of MBZ and LVM. For the simultaneous equation method, the estimation of MBZ was carried out at 286nm (ZCP of LVM) and of LVM at 245nm (ZCP of MBZ). The linearity was obtained in the concentration ranges of 2-12 μg/ml for MBZ and 3-18 μg/ml for LVM with a correlation coefficient (r2) value greater than 0.998. The % RSD value for intraday & interday precision were less than 2. The detection limit and quantification limit were found to be 0.441 μg/ml and 0.145 μg/ml for MBZ and 0.681 μg/ml and 0.225 μg/ml for LVM, respectively. All the validation parameters were performed as per the ICHQ2 (R1) guidelines. The estimation study on marketed tablet preparation was carried out, results were 99.05% for MBZ and 98.78% for LVM. So, the developed method could be applied for the routine quality control analysis of MBZ and LVM in combined tablet formulation.
Cite this article:
Vaibhav Jain;, Rajkumar Chauhan; B. P. Nagori; Sabir Anis; Ranjan Kumar Singh. Method Development and its Validation for Simultaneous estimation of Levamisole Hydrochloride and Mebendazole as API and in Combination in Tablet Dosage Form by Simultaneous Equation Method of UV Spectrophotometry. IJRPAS, May-June 2024; 3(3): 167-176.
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